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This Concept Map, created with IHMC CmapTools, has information related to: Epilepsy Paper, Hippocampus conclusion Also see in the soma, generation of antibodies used in Western Blot, RNA and Protein levels Localized in Caudal area of brain, Immunohistochemistry and Immunofluerescence Histochemistry Detects antibody/antigen complexes, Figure 2 took EcoG to see seizures, Hippocampus conclusion Nav1.1 localizes in AISs of PV interneurons, Southern bolt determined correct cells Homologous Recombination in embryonic stem cells, Immunohistochemistry and Immunofluerescence Histochemistry Generate Western Blot, Hypothesis: impaired function of PV inhibitory circuit can lead to epileptic seirsures Generation of Scn1a knock-in mouse, Created a vector used dCTP-labeled DNA in exaon 21 (probe), RNA and Protein levels Results Only in +/+ not Rx/Rx mice, Loss-of-function in Nav1.1 Mutations lead to decrease in inhibition, decrease in inhibition leads to inability to excite neruons, Western Blot determines if the proper protein made, Paravalbumin-positive interneurons type of fast-spiking interneuron, In Situ Hybridization Next generation of antibodies, untable gait, seizures, premature death in Rx/Rx results in Figure 2, Creation of premature stop codon caused by the Na channels messed up in inhibitory neurons, Loss-of-function in Nav1.1 determined RNA levels Nothern Blot, Western Blot Results in Figure 3