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This Concept Map, created with IHMC CmapTools, has information related to: Biotechnolopgy Process cMap, Centrifuge Solid Discard the Solid, Freezer Seedstock Aseptic Hood, Pump to Next Process High Salt Eluate Tangential-Flow Filtration (Ultrafiltration Membrane) Ultrafiltration (concentartion), ++Beads in the Chromatography Column GFP Solution UV Optical Sensor, Colum Chromatography for Difilltration [Anion-Exchange Chromatography] (exchanging buffers in the Lysate) --GFP is attracted to the ++ Beads ++Beads in the Chromatography Column, Tangential-Flow Filtration (Ultrafiltration Membrane) Ultrafiltration (concentartion) Eluate Ammonium Sulfate Filltration, 0.22 Micron Filter (Remove the Solids) Bacteria is 1 Micron in diameter Clarified Lysate (Biological Soup) Lysate Transfer Vessel, Chromatagraphy Machine Lysate 0.45 Micron Pre-Filter, ++Beads in the Chromatography Column GFP Solution pH Sensor, ++Beads in the Chromatography Column Non GFP Solution Waste, .22 Micron Filter Move to FIll/Finish Freeze Dry Package, Chromatagraphy Machine Lysate Air Meter, NaCl Solution to Realese GFP from Beads Eluate (Prouduct) UV Optical Sensor (Checking for GFP), Lysis Lysate Recovery, Centifuge Suspended Cleaned Cells Homoginizer (Cell Disrupter), Homoginizer (Cell Disrupter) After Loop Ruptered Cells, Chromatagraphy Machine Lysate Conductivity Sensor, Tangential-Flow Filtration (Ultrafiltration Membrane) Ultrafiltration (concentartion) Recycle Untill Concentrated, Homoginizer (Cell Disrupter) 1st Time Ruptered Cells, Tangential-Flow Filtration (Ultrafiltration Membrane) Ultrafiltration (concentartion) Recycle Untill Concentarted