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This Concept Map, created with IHMC CmapTools, has information related to: Plant Cmap, Pump to next process Pressure Sensor, Column Chromatography for Diafiltration Non GFP Solution is waste pH Sensor, Centrifuge Discard solid, Suspended Cells in Cleaning Solution Centrifuge, Discard Liquid Cell Paste (Solid) Suspended Cells in Cleaning Solution, Flow Meter part of Clarified Lysate in Transfer Vessel, Aseptic Hood Broth Bioreactor, Column Chromatography for Diafiltration --GFP is attracted to the ++beads ++Beads in the Chromatography Column, 0.22 Micron Filter Clarified Lysate Lysate Transfer Vessel, Suspended Cleaned Cells Homogenizer, NaCl Solution to Release GFP from Beads Collection Vessel Concentrate GFP, Concentrate GFP High Salt Eluate Pump to next process, Homogenizer Ruptured Cells Centrifuge, Conductivity Sensor part of Clarified Lysate in Transfer Vessel, Column Chromatography for Diafiltration Non GFP Solution is waste UV Optical Sensor (Checking for GFP), Bioreactor Glucose analyzer, Air meter part of Clarified Lysate in Transfer Vessel, Lysate Transfer Vessel ???? Clarified Lysate in Transfer Vessel, ++Beads in the Chromatography Column Elution NaCl Solution to Release GFP from Beads, Lysate(with Product GFP) 0.22 Micron Filter