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This Concept Map, created with IHMC CmapTools, has information related to: Biotechnology Facility_DTS BT F 2018, Centrifuge 2nd time Homogenizer, Purification Clarified Lysate in Transfer Vessel .45 Micron Pre-Filter, NaCl Solution to Release GFO from Beads Eluate (Product) UV Optical Sensor (Checking for GFP), Pump to next process Pressure Sensor Tangential-Flow Filtration (Ultrafiltration Membrane) Ultrafiltration (Concentration), pH Sensor Elution NaCl Solution to Release GFO from Beads, UV Optical Sensor (Checking for GFP) Collection Vessel Concentrate GFP, Bioreactor Broth Broth Tank, Permanent (Waste) Recycle until concentrated Retenate (Product), Purification Clarified Lysate in Transfer Vessel Flow Meter, Purification Clarified Lysate in Transfer Vessel Pressure Sensor, Ammonium Sulfate Second TTF Process New Buffer, Retenate (Product) Recycle until concentrated Permanent (Waste), Freezer Feed Stock Aseptic Hood, Cleaned Cell Paste Suspended Cleaned Cells Homogenizer, Cell Paste Suspended Cells in Cleaning Solution Cleaned Cell Paste, New Buffer Final Filter .22 Micron Filter, Cell Paste Suspended Cells in Cleaning Solution Centrifuge, Aseptic Hood Broth Bioreactor, ++ Beads in Chromatography Column Non GFP solution is waste Conductivity Sensor, Purification Clarified Lysate in Transfer Vessel Inlet Pump