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This Concept Map, created with IHMC CmapTools, has information related to: Biotechnology Facility_KJ BF 0920, Pump to next process Pressure sensor Tangential-Flow-Filtration, Pump to next process High Salt Elute Tangential-Flow-Filtration, Pump to next process High Salt Elute Ammonium Sulfate, Purification Clarified Lysate in Transfer Vessel Pressure sensor, Ammonium Sulfate Second TFF process New Buffer, Purification Clarified Lysate in Transfer Vessel .45 Micron Pre-Filter, pH Sensor Elution NaCl Solution to relase GFP from Beads, Air meter Filtered Clarified Lysate(pH 8.0) Column Chromatography for Diafiltration, Fermentation seed stck Freezer, Homogenizer(Cell Disrupter) Ruptured Cells Centrifuge, Bioreactor broth broth tank, Purification Clarified Lysate in Transfer Vessel Conductivity Sensor, Freezer feed stock Aseptic Hood, Discard the liquid cell paste Centrifuge, Recovery Lysate .22 Micron Filter(remove the solid) Bacteria is i micron in diameter, Discard the liquid Suspended Cells in Cleaning solution Centrifuge, Aseptic Hood Broth Bioreactor, New Buffer Final Filter .22 Micron Filter, Ammonium Sulfate Permanent New Buffer, UV Optical Sensor(Checking for GFP) Collection Vessal Concentrate GFP