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This Concept Map, created with IHMC CmapTools, has information related to: 01. DNA cloning, must know sequence before starting allows for Fine-tuned sequence targeting, libraries either cDNA, repair deficient bacteria implies mutation carried forward following replication, synthetic primers implies must know sequence before starting, DNA CLONING TECHNIQUES may begin with DNA, DNA first Break DNA into small segments, cDNA from mRNA, Manipulated examples Site-directed mutagenesis, RNA olden days mRNA, libraries either genomic libraries, RNA reverse transcriptized to RNA-cDNA complex, separate strands via heat, cDNA implies no promoter sequences, Break DNA into small segments introduce into plasmid, Site-directed mutagenesis whereby change 1 basepair, insert cDNA into plasmid, PCR First separate strands, restriction enzyme targe sites which can be used to insert cDNA, genomic libraries from DNA, plasmid properties of a good plasmid Selectable markers