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This Concept Map, created with IHMC CmapTools, has information related to: Chapter 20, cloning vector are easily isolated, Genetically modified when placed in organism GMO, restriction enzymes each specific, restriction enzymes rocognizes restriction site, clone useful purposes make copies, PCR is quicker, Ti plasmid introduces new genes, DNA yielding restriction fragments, clone containing 10^5 cells colony, restriction enzymes cut foreign DNA, plasmids original called cloning vector, restriction enzymes protect bacterial cell, evolutionary relationships between species, yeast artificial chromosomes can carry BAC, gene afflicted ones may be altered called gene therapy, genome sequencing comparisons help us determine evolutionary relationships, PCR 3-step cycle extension, humans is a mapping project Human Genome Project, restriction fragment analysis useful for comparing 2 alleles, clone of identical cells