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This Concept Map, created with IHMC CmapTools, has information related to: Biotechnology Processes, Purification Clarified Lysate in Transfer Vessel Air Meter, Purification Clarified Lysate in Transfer Vessel Inlet pump, Bioreacter Broth Tank, Ammonium Sulfate Recycle until concentrated New Buffer, Purification Clarified Lysate in Transfer Vessel .45 Micron Pre-Filter, Discard the Liquid Cell Paste (Solid) Centrifuge, Centrifuge Cleaned Cell Paste Homoginizer (Cell Disrupter), Bioreacter Spectrophotometer, Purification Clarified Lysate in Transfer Vessel Pressure Sensor, Bioreacter Glucose Analyzer, Bioreacter pH meter, Lysis Broth Centrifuge, Purification Clarified Lysate in Transfer Vessel Column Chromatography for Diaflitration, Purification Clarified Lysate in Transfer Vessel Conductivity Sensor, New Buffer Second TFF process .22 Micron Filter, Centrifuge Discard the Solid Lysate, Recovery Lysate (Liquid with Product GFP .22 Micron Filter (Remove the solids) Bacteria is 1 micron in diameter, Column Chromatography for Diaflitration Filtered Clarified Lysate (pH 8.0) ++ Beads in the Chromatography Column, ++ Beads in the Chromatography Column --GFP is attracted to the ++GFP UV Optical Sensor (Checking for GFP), Pump to next process Pressure Sensor